micro biology

Chapter 11 – Physical and Chemical Control of Microbes

Part I.  Define the following terms:

  1. Sterile
  2. Disinfect
  3. Antiseptic
  4. Decontamination
  5. Microbicidal
  6. Microbialstatic

Part II.  Complete the chart below using Chapter 11 as a reference for Physical Agents.

Physical methods of ControlType of Agent Dry/wet Hot/cold/ radiation(ioning/nonionzing)Kind of agent (sterilant, disinfectant, antiseptic)Recommended uses (Where is this used?)
1 Autoclave  Wet heat (steam under pressure)sterilantLaboratory glassware, intstruments, dressings, etc
2     
3     
4     
5     
6     

Part III.  Complete the chart below using Chapter 11 as a reference for Chemical Agents.

Chemical Methods of ControlType of agent (halide, alcohol, surfactant, etc)Target (what is killed or inhibited AND kind of agent (sterilant, disinfectant, antiseptic)Recommend uses (Where is this used?)
1 chlorine and derivatives  HalideSporicidal/intermediateBroad uses in disinfectants; drinking water, swimming pools
2     
3     
4     
5     

Part IV-Questions:

1. List different microbial types in order of least to most resistance with regard to microbial control agents.

2. Compare types of radiation (X-rays, UV light) with regard to anti-microbial activity. 

3. Know examples of when you would use the various types of physical and chemical methods of control.

Part V-Around your house or work

1. Find two disinfectants around the house, if you don’t have any do a search to find ones that you would buy.  Fill in the chart below with two different active agents.  Give the name, the active agent with concentration, the spectrum of activity (what organisms it kills), and the places it can be used.

CharacteristicsDisinfectant #1Disinfectant #2
Name of Agent  
Active Ingredients (agents)  
Concentration of active agents  
Spectrum of Activity (what it kills)  
Is it static or cidal?  
Where can it be used?  

2.  Find two antiseptics around the house, if you don’t have any do a search to find ones that you would buy.  Fill in the chart below.  The two must have different active agents.  Give the name, the active agent with concentration, the spectrum of activity (what organisms it kills), and the places it can be used.

CharacteristicsAntiseptics #1Antiseptic  #2
Name of Agent  
Active Ingredients (agents)  
Concentration of active agents  
Spectrum of Activity (what it kills)  
Is it static or cidal?  
Where can it be used?  

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